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<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
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<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
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<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
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<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
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<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
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<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
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ATCC wild type deleted nucleotides chr5 pta 125816 sequence
<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
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gene edited animals line number cd163 allele 1 cd163 allele 2 atcc deposit no 2 wild type deleted nucleotides chr5 pta 125814 sequence  (ATCC)
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ATCC gene edited animals line number cd163 allele 1 cd163 allele 2 atcc deposit no 2 wild type deleted nucleotides chr5 pta 125814 sequence
<t>Nucleotide</t> sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.
Gene Edited Animals Line Number Cd163 Allele 1 Cd163 Allele 2 Atcc Deposit No 2 Wild Type Deleted Nucleotides Chr5 Pta 125814 Sequence, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nucleotide sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.

Journal: Microbial Cell

Article Title: Overcoming phagocytosis resistance of hypervirulent Klebsiella pneumoniae by directly targeting capsules

doi: 10.15698/mic2026.02.870

Figure Lengend Snippet: Nucleotide sequence of the rmpADC operon (A) and manC ORF (B) obtained from the genome of K. pneumoniae ATCC43816. (A) The rmpA ORF is shown in red, the rmpD in blue, and the rmpC in purple. (B) The manC ORF is shown in red. Primer sequence used for PCR amplification is shown in bold. (C) Agarose gel electrophoresis of PCR products for rmpADC and manC in each strain.

Article Snippet: The nucleotide sequences of each PCR product were directly sequenced by using the ABI 3500XL genetic analyzer (Applied Biosystems, Foster City, CA, USA) in accordance with the protocol of the BigDye Terminator v3.1 Cycle Sequencing Kit method (Thermo Fisher Scientific, cat. no. 4336919).

Techniques: Sequencing, Amplification, Agarose Gel Electrophoresis